Enzyme catalase coursework

This is because the larger the scale, the lower the percentage error. However, some oxygen was displaced in the gas syringe and I had to solve this by subtracting this small amount from the volumes produced in each of the reactions.

Effect of Substrate Concentration on the Rate of Activity of Catalase

Hence the percentage error was: I have drawn a line of best fit to clearly illustrate this trend. As hydrogen peroxide enters the active siteit interacts with the amino acids Asn asparagine at position and His75causing a proton hydrogen ion to transfer between the oxygen atoms.

This could have affected my results for several reasons. In these individuals the pathogen survives and becomes a chronic infection.

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Lesson organisation You could run this investigation as a demonstration at two different concentrations, or with groups of students each working with a different concentration of hydrogen peroxide.

Set out in brown bottles Note 1. Other catalase-positive organisms include ListeriaCorynebacterium diphtheriaeBurkholderia cepaciaNocardiathe family Enterobacteriaceae CitrobacterE.

So therefore a gradual decrease in temperature will mean an unfair test as temperature was a variable that should be controlled as the rate of reaction is dependant on the temperature. Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell.

Action Of Enzyme Catalase On Hydrogen Peroxide - Lab Report Example

It was very hard to insert the small 5cm3 beaker into the conical flask, and when it came to tipping it over, some of the substrate was still trapped inside the beaker.

I believe that my results were also relatively reliable because as the concentration decreased the volume of oxygen produced also decreased. This means that in my results, the volume of gas produced in the first 5 seconds may have been higher than it should have been if I had used exactly 0. This is because I did not use specific equipment which would have maintained the temperature, which would have allowed me to gain more accurate and reliable result.

This beetle has two sets of liquids that are stored separately in two paired glands. The increased oxidative stress that occurs with aging in mice is alleviated by over-expression of catalase. Another factor which was hard to measure was the volume of gas produced, because some of the higher concentration reactions were very fast, so it was hard to read the correct values every time.

Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

Evaluation Overall, I believe my experiment went well and that I gained sufficient results because I repeated each concentration three times and investigated eight concentrations in total. Symptoms are mild and include oral ulcers. The hand holding the tube is then tapped on the bench, moving the hydrogen peroxide down until it touches the bacteria.

This very important as the rate of reaction Enzyme catalase coursework dependant on temperature hence I will have to make sure that temperature is controlled.

I believed this was accurate, but upon reflection, using a pipette would have been much more accurate as pipettes have a much lower apparatus error than volumetric flasks.

I will then work out the gradient of the two concentrations and plot them on a rate of reaction graph along with the other concentrations. For example, if there were more molecules of yeast, the rate of reaction would increase because there would be more collisions between enzyme and substrate molecules.

Discs of potato react too slowly. Hence by doing this the volume of gas escaping through the gap will be much lower, As result of this a more accurate reading of the actual volume of oxygen produced.Enzymes require an optimal range of temperature and pH in order to have an effective enzyme-catalyzed reaction.

The enzyme used in this experiment was catalase, whose function is to prevent the accumulation of toxic levels of hydrogen peroxide formed as a by-product of cellular processes. Catalase is an enzyme that converts hydrogen peroxide into water and oxygen gas. Enzymes are protein molecules that are composed of subunits called amino acids.

Amino acids are similar to links in a chain, while protein is similar to the chain itself. An enzyme that is also naturally produced by the body is catalase, and this is what the liver uses to break down hydrogen peroxide.

When certain factors come into play, temperature being one, catalase may not work as well. The enzyme catalase is responsible for the decomposition of Hydrogen Peroxide into Oxygen and Water (Palmer, Bonner, ).

Previous researchers have noticed that enzymes, like catalase, perform best at their optimal temperature. This is because as enzyme concentration increases then there is a greater probability of collision between catalase and hydrogen peroxide. Hence more products produced in a given time.

However the rate of reaction is constant when all substrate, hydrogen peroxide molecules have occupied the enzyme, catalase active site. Catalase is an enzyme present in the cells of plants, animals and aerobic (oxygen requiring) bacteria. It promotes the conversion of hydrogen peroxide, a.

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Enzyme catalase coursework
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